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Recent Publications using our Fan less, Vibration free Microscope Incubation Technology



Custom TIRF/Spining-Disk, Nikon Ti, Yokogawa Spinning Disk

MRC Laboratory of Molecular Biology, Cambridge, UK


Macromolecular Condensation Buffers Intracellular Water Potential

Optimum protein function and biochemical activity critically depends on water availability because solvent thermodynamics drive protein folding and macromolecular interactions1. Reciprocally, macromolecules restrict the movement of 'structured' water molecules within their hydration layers, reducing the available 'free' bulk solvent a nd therefore the total thermodynamic potential energy of water, or water potential. Here, within concentrated macromolecular solutions such as the cytosol, we found that modest changes in temperature greatly affect the water potential, and are counteracted by opposing changes in osmotic strength.

"Dr E. Derivery at al."
- October 2023

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Live Cell Imaging Raman Spectroscopy Cambridge

University of Cambridge, University of Oviedo, Cancer Research UK Cambridge Institute


Evaluation of Label-Free Confocal Raman Microspectroscopy for Monitoring Oxidative Stress In Vitro in Live Human Cancer Cells

Understanding the impact of free radicals and antioxidants in cell biology is vital; however, noninvasive nonperturbative imaging of oxidative stress remains a challenge. Here, we evaluated the ability of label-free Raman spectroscopy to monitor redox biochemical changes in antioxidant (N-acetyl-L-cysteine, NAC) and pro-oxidant (tert-butyl hydroperoxide, TBHP) environments. Cellular changes were compared to fluorescence microscopy using CellROX Orange as a marker of oxidative stress.

"Jakub Maciej Surmacki, Isabel Quiros-Gonzalez and Sarah Elizabeth Bohndiek"
- Febuary 2022

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TIRF Super Resolution Imaging

University of Paris, University of Cambridge, Grenoble

Nikon TiE, Cairn TIRF, Yokogawa Spinning Disk


The Arp1/11 Minifilament of Dynactin Ignites the Endosomal Arp2/3 Complex.

Dendritic actin networks grow in an autocatalytic manner starting from the uncapped minifilament of Dynactin.

"Fokin AI, Violaine David, Ksenia Oguievetskaia, Emmanuel Derivery, Caroline E. Stone, Luyan Cao, Nathalie Rocques, Nicolas Molinie, Véronique Henriot, Magali Aumont-Nicaise, Maria-Victoria Hinckelmann, Frédéric Saudou, Christophe Le Clainche, Andrew P. Carter, Guillaume Romet-Lemonne, Alexis M. Gautreau."
- May 2020

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TIRF Super Resolution Imaging

Manchester, Geneva, Cambridge, Edinburgh, London

Nikon TiE, Cairn TIRF, Yokogawa Spinning Disk


Compensatory Ion Transport Buffers Daily Protein Rhythms to Regulate Osmotic Balance and Cellular Physiology.

Between 6-20% of the cellular proteome is under circadian control to tune cell function with cycles of environmental change. For cell viability, and to maintain volume within narrow limits, the osmotic pressure exerted by changes in the soluble proteome must be compensated.

"Stangherlin A, Wong D, Barbiero S, Watson J, Beale A, Goh C, Feord H, Maywood E, Bartolami E, Matile S, Lequeux N, Pons T, Day J, Van Ooijen G, Derivery E, Edgar R, Newham P & O’Neill J."
- May 2020

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Optical Tweezer- Custom Designed Microscope

CIML Marseille


Independent Synchronized Control and Visualization of Interactions between Living Cells and Organisms

"Vincent Rouger, Guillaume Borde, Carole Couillault, Serge Monnere, Sebastien Mailfer, Jonathan J. Ewbank, Nathalie Pujo, Didier Margue"
- May 2014

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Single Molecule Imaging of Yeast S.pombe at 30oC

Sussex University

Olympus IX71, Photometrics Camera


Single-Molecule Live Cell Imaging of the Smc5/6 DNA Repair Complex.

The Smc5/6 complex is involved in various DNA transactions and is best known for ensuring the fidelity of homologous recombination.

"Thomas J. Etheridge1*, Desiree Villahermosa1, Anja Irmisch1,3, Adam T. Watson1, Alex Herbert1, Hung Q. Dang1,4, Mark A. Osborne2, Antony W. Oliver1, Antony M. Carr1 and Johanne M. Murray"
- June 2020

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Confocal Imaging Zeiss LSM880 with Airyscan

Sussex University


Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.

Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis.

"Hégarat, N., Crncec, A., Suarez Peredo Rodriguez, M., Echegaray Iturra, F., Gu, Y., Busby, O., Lang, P., Barr, A., Bakal, C., Kanemaki, M., Lamond, A., Novak, B., Ly, T. and Hochegger, H., 2020. Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. The EMBO Journal, 39(11)."
- March 2020

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Olympus IX81, 3i Imaging, Yokogawa Spinning Disk

Sussex University


Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.

"Tom Stiff, Fabio R. Echegaray-Iturra, Harry J. Pink, Alex Herbert, Constantino Carlos Reyes-Aldasoro, Helfrid Hochegger."
- May 2020

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